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KMID : 0361020020450090873
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Korean Journal of Otolaryngology - Head and Neck Surgery
2002 Volume.45 No. 9 p.873 ~ p.877
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Budesonide Down-regulates IL-1β-Mediated MUC2/MUC5AC Genes Expression and Mucin Secretion in Human Airway Epithelial Cells
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김용대/Yong Dae Kim
조중석/장근영/신재흔/송시연/윤석근/Jung Suck Cho/Keun Young Chang/Jae Heun Sin/Si Youn Song/Seok Keun Yoon
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Abstract
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Background and Objectives: Mucus hypersecretion is a hallmark of many respiratory inflammatory diseases such as asthma, bronchitis and sinusitis. While the current therapeutic pharmacological approaches to reducing mucus hypersecretion are
limited, clinical studies have suggested that glucocorticoids reduce mucus secretion in patients with airway disease. However, the effect of glucocorticoids on mucus hypersecretion is not clear. Recently, we observed that IL-1β induces MUC2 gene
expression and mucin secretion in a previous experiment. This study was designed to investigate the effects of budesonide on the IL-1β-mediated MUC2/5AC genes expression and mucin secretion.
Materials and Method: We observed the steady state mRNA level of MUC2/5AC genes using RT-PCR and mucin protein using immunoassay method in cultured human airway NCI-H292 epithelial cells.
Results: Budesonide attenuated IL-1β-mediated MUC2/5AC gene expression as well as mucin secretion. The attenuated effect of budesonide was in a dose-dependent pattern. This attenuated effect of budesonide was blocked by glucocorticoid
receptor
antagonist, RU-486.
Conclusion: This result suggests that budesonide suppresses the IL-1β-mediated MUC2/5AC genes expression and mucin secretion via blockage of glucocorticoid receptor.
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